Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.216613 |
Chromosome: | chromosome 6 |
Location: | 4252780 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g278246 | 5'UTR_intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCATTGTATTAGCTTTAAACCAGACAGT |
Internal bar code: | TGGCAGGTTGATTTAGGTGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 962 |
LEAP-Seq percent confirming: | 99.6531 |
LEAP-Seq n confirming: | 5171 |
LEAP-Seq n nonconfirming: | 18 |
LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCCTGACGTCAAGTACCCG |
Suggested primer 2: | CCGATGGAGCTTAAGCAGTC |