Insertion junction: LMJ.RY0402.216628_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.216628
Chromosome:chromosome 12
Location:7676550
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g554250 LPB1 (1 of 1) PTHR11952:SF8 - UDP-GLUCOSE PYROPHOSPHORYLASE 3; Low Photochemical Bleaching protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAATTGTGCCCAAGACGCAACCTCGGCCC
Internal bar code:GGGATGGCCATCCTCTCGCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:296
LEAP-Seq percent confirming:84.6512
LEAP-Seq n confirming:364
LEAP-Seq n nonconfirming:66
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTTGCTCGATTCTCCAAC
Suggested primer 2:CGGTTTACTTTGGGCTGGTA