| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216629 |
| Chromosome: | chromosome 8 |
| Location: | 898154 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361450 | (1 of 1) IPR008867//IPR009836 - Thiazole biosynthesis // Protein of unknown function DUF1399 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACCCGGTCGTGAGCACCGAGGGGCTGGC |
| Internal bar code: | GGATACGGTAAGGTAGGCTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 262 |
| LEAP-Seq percent confirming: | 97.8355 |
| LEAP-Seq n confirming: | 226 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGCTTACCCTTTGCCCTT |
| Suggested primer 2: | GGAGGACTCAAATTACGCCA |