Insertion junction: LMJ.RY0402.216631_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.216631
Chromosome:chromosome 3
Location:3361405
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g166700 FOR1 (1 of 3) PTHR23213//PTHR23213:SF214 - FORMIN-RELATED // SUBFAMILY NOT NAMED; Formin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAGGTTTGGTCAAGGGGTTGTGAGCCGA
Internal bar code:ACAACGGCATAGGGATCGTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1003
LEAP-Seq percent confirming:99.3069
LEAP-Seq n confirming:11033
LEAP-Seq n nonconfirming:77
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTGCACTGGAAGAAGCTG
Suggested primer 2:ATACCGTGCAGAACCACCTC