| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216634 |
| Chromosome: | chromosome 3 |
| Location: | 2486085 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159650 | EFG6 | (1 of 1) PTHR23115//PTHR23115:SF96 - TRANSLATION FACTOR // SUBFAMILY NOT NAMED; Putative organellar translation elongation factor EFG/EF2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGCGCAGGAAAACATACTCTGGGGGGT |
| Internal bar code: | TCCAGCCGGCTAGCTCGTTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 670 |
| LEAP-Seq percent confirming: | 99.6457 |
| LEAP-Seq n confirming: | 7875 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGATGCCTGTGGTTAGGT |
| Suggested primer 2: | TAGCTGTCTGGTGCCATCTG |