Insertion junction: LMJ.RY0402.216635_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.216635
Chromosome:chromosome 1
Location:7015042
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g050616 5'UTR_intron
Cre01.g050624 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTTATGCCTTGCAGCCGCATGGGCGCAG
Internal bar code:TACCGAATCGGGCAACATTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1039
LEAP-Seq percent confirming:99.4155
LEAP-Seq n confirming:2211
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGCACTACCCACGGAATG
Suggested primer 2:GCGCTTCTGCTCTAGCTTGT