Insertion junction: LMJ.RY0402.216636_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.216636
Chromosome:chromosome 6
Location:6755208
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g294700 DIV152 (1 of 6) PF01477 - PLAT/LH2 domain (PLAT) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACAACATACTTGCCGCGCCAAAGAGCGGG
Internal bar code:TTGTTCGTCAACGTGGGATAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:689
LEAP-Seq percent confirming:99.2815
LEAP-Seq n confirming:1520
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AACCCACTGATCTTGTTGGC
Suggested primer 2:CTCCTGCACCTGGCTAGAAG