| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.216649 |
| Chromosome: | chromosome 9 |
| Location: | 5472211 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400219 | (1 of 32) 3.1.1.3 - Triacylglycerol lipase / Triglyceride lipase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGTGGACGTTACAGAGAGGAGCGTGTCT |
| Internal bar code: | CCACTACAACTTGTATGACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 633 |
| LEAP-Seq percent confirming: | 99.8996 |
| LEAP-Seq n confirming: | 995 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGGGATCGTCAGTAGGA |
| Suggested primer 2: | TGGTTCTCGGTTTCCTGTTC |