Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.216683 |
Chromosome: | chromosome_10 |
Location: | 4603171 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g452750 | TPT16 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGGCGGATGCCGGTGAAGCACGTGACATT |
Internal bar code: | CTGCCTGCAGCCTGTTCTGCCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 682 |
LEAP-Seq percent confirming: | 99.5817 |
LEAP-Seq n confirming: | 7142 |
LEAP-Seq n nonconfirming: | 30 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACTCCGTATCCGTTGGTCT |
Suggested primer 2: | GTGATGAGGGGACGTGAGTT |