| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.216693 |
| Chromosome: | chromosome 6 |
| Location: | 2983835 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273400 | TMC2 | putative tRNA (cytosine-5)-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAACGGCCAGGGCGGCGGCAGGCAAAGTC |
| Internal bar code: | TACGTCAAGCCTCTCGGGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 692 |
| LEAP-Seq percent confirming: | 99.8912 |
| LEAP-Seq n confirming: | 918 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATTGAGGTTGGCCATGTA |
| Suggested primer 2: | CGTGTTGCTTGACCTACGAA |