| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216701 |
| Chromosome: | chromosome 17 |
| Location: | 3209074 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g722250 | RRM14 | Putative rRNA (guanine-N2-)-methyltransferase; (1 of 1) 2.1.1.173//2.1.1.264 - 23S rRNA (guanine(2445)-N(2))-methyltransferase // 23S rRNA (guanine(2069)-N(7))-methyltransferase / 23S rRNA m(7)G(2069) methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCACCAAACGGCTCACTACAAACGCACT |
| Internal bar code: | TCGGACCACCGTATGTTTCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 222 |
| LEAP-Seq percent confirming: | 87.5811 |
| LEAP-Seq n confirming: | 21460 |
| LEAP-Seq n nonconfirming: | 3043 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGATTGCTTACACTCGGCT |
| Suggested primer 2: | GGTACCAGATACGCGACCTC |