| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216718 |
| Chromosome: | chromosome 3 |
| Location: | 5814879 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189100 | (1 of 1) PTHR31606:SF1 - WBP2 ORTHOLOG, ISOFORM E | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCGGCACGTGCCTTCCCAGCTACCTACT |
| Internal bar code: | CAAAATGCGGAGAGTCGATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 618 |
| LEAP-Seq percent confirming: | 73.264 |
| LEAP-Seq n confirming: | 5212 |
| LEAP-Seq n nonconfirming: | 1902 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACCAGTCAACTGTCAGCA |
| Suggested primer 2: | AGCGTGTGGACACTAATCCC |