| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.216728 |
| Chromosome: | chromosome 3 |
| Location: | 2090266 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156750 | CCT5 | T-complex protein 1, epsilon subunit; (1 of 1) K09497 - T-complex protein 1 subunit epsilon (CCT5) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTCCTGTGATTGATGTAACACGAGCCAG |
| Internal bar code: | CTTATATGCGGCAGCTCCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 891 |
| LEAP-Seq percent confirming: | 99.562 |
| LEAP-Seq n confirming: | 11365 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGGAAGAAGGAGCTGTG |
| Suggested primer 2: | AAATGGTCCGCTCATGAATC |