| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216747 |
| Chromosome: | chromosome 3 |
| Location: | 4372220 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175300 | FAS14,FLA9 | (1 of 10) PTHR10900 - PERIOSTIN-RELATED; FAS1 domain containing protein; | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCGATCCTGCTGGCTGGTGCGGGGCTTC |
| Internal bar code: | TACTAGTCCGGGTTCCGACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 827 |
| LEAP-Seq percent confirming: | 97.2822 |
| LEAP-Seq n confirming: | 9736 |
| LEAP-Seq n nonconfirming: | 272 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACTGGTGAGTTCCAGGGG |
| Suggested primer 2: | GGGCCAGACACTGAGATGTT |