| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216750 |
| Chromosome: | chromosome 2 |
| Location: | 6580913 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g117150 | (1 of 1) PTHR23077:SF10 - PACHYTENE CHECKPOINT PROTEIN 2 HOMOLOG | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTAAGATGTTGATTGCAATCAGCCCATCC |
| Internal bar code: | CCGGCGGGCAAGTCGCAGCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 99.8187 |
| LEAP-Seq n confirming: | 2753 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGGAAGACCACAAGGTG |
| Suggested primer 2: | TCGGGTGGTAGTAGTCTGGG |