| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.216768 |
| Chromosome: | chromosome_16 |
| Location: | 3812373 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g689600 | FAP73 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCAGCTCCTCCAGCACCTCTGTAAGTTGC |
| Internal bar code: | ACACTGAGGAAACCGTCGTATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1072 |
| LEAP-Seq percent confirming: | 98.4942 |
| LEAP-Seq n confirming: | 4775 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCTGGTTGTTGAGGTTT |
| Suggested primer 2: | AACACACAGAAGCAAACCCC |