| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.216774 |
| Chromosome: | chromosome 6 |
| Location: | 8114914 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304850 | (1 of 1) PF04424 - Protein of unknown function (DUF544) (DUF544) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGGAGCAATGGGAGGGCGGAAAAGGTG |
| Internal bar code: | GCTACACCGTTTAAGGGTCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 153 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 805 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGTGTGAGCGCTATGTG |
| Suggested primer 2: | GTCTCCACCTCCACCGTTAG |