| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.216804 |
| Chromosome: | chromosome 17 |
| Location: | 4849185 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g734134 | 3'UTR | ||
| Cre17.g734142 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCGTGCCAGGATAGATGTAATGCGTGGG |
| Internal bar code: | CTGTGGATATCAGGTGAGAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 205 |
| LEAP-Seq percent confirming: | 70.9677 |
| LEAP-Seq n confirming: | 132 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGCATTTTCAGCAGCCAT |
| Suggested primer 2: | TGGTCGAGTTGATGTTTCCA |