Insertion junction: LMJ.RY0402.216832_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.216832
Chromosome:chromosome 6
Location:6608986
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g293650 (1 of 26) IPR013026//IPR019734 - Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCTCCGTGTTAAAGAGGCAAAATGAGG
Internal bar code:CTGTCTGATACGGGCCAATTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:267
LEAP-Seq percent confirming:85.1823
LEAP-Seq n confirming:12216
LEAP-Seq n nonconfirming:2125
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:AACCAAGCTGAACTAGCGGA
Suggested primer 2:TTGGGGTAGAACACGGACTC