| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216842 |
| Chromosome: | chromosome 3 |
| Location: | 8931742 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g208609 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCTGTCGGCGCGTTGGGCTGTTGCCGCA |
| Internal bar code: | TGTCGCGTCGCTAAAGGCTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 739 |
| LEAP-Seq percent confirming: | 98.3656 |
| LEAP-Seq n confirming: | 11375 |
| LEAP-Seq n nonconfirming: | 189 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTCGTGTGGATGTTTTT |
| Suggested primer 2: | GCACATGATACAACGATGCC |