Insertion junction: LMJ.RY0402.216850_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.216850
Chromosome:chromosome 12
Location:4758678
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g524000 SUC1,GTR7 (1 of 1) 2.4.1.14 - Sucrose-phosphate synthase / UDP-glucose-fructose-phosphate glucosyltransferase; Sucrose-phosphate synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACACACACGCACACACACGCACATGCA
Internal bar code:GAGGGCAAAATCCGGGTCACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:116
LEAP-Seq percent confirming:93.9317
LEAP-Seq n confirming:8854
LEAP-Seq n nonconfirming:572
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACTCTTACACACGCCG
Suggested primer 2:GGGTGTTGACTCGTCCACTT