Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.216853 |
Chromosome: | chromosome_3 |
Location: | 6663287 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g197250 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GACCACAGCCACAGTCACAGCCACAGCCGC |
Internal bar code: | GGCGGGCAGCGAGGGTCGACCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 783 |
LEAP-Seq percent confirming: | 92.8477 |
LEAP-Seq n confirming: | 701 |
LEAP-Seq n nonconfirming: | 54 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACGGACATTACGGTGCTCA |
Suggested primer 2: | TAGCCGCAGTATGTCACCTG |