| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.216853 |
| Chromosome: | chromosome 3 |
| Location: | 6663287 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197250 | (1 of 1) PTHR13948//PTHR13948:SF25 - RNA-BINDING PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCACAGCCACAGTCACAGCCACAGCCGC |
| Internal bar code: | GGCGGGCAGCGAGGGTCGACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 783 |
| LEAP-Seq percent confirming: | 92.8477 |
| LEAP-Seq n confirming: | 701 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGGACATTACGGTGCTCA |
| Suggested primer 2: | TAGCCGCAGTATGTCACCTG |