| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.216863 |
| Chromosome: | chromosome 12 |
| Location: | 8743865 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g545800 | ROC55,ROC11 | Rhythm Of Chloroplast 55; (1 of 24) IPR006553 - Leucine-rich repeat, cysteine-containing subtype | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAACAGCCATGACAGCAACATCCTCATT |
| Internal bar code: | GTGATCGAGCGAAGGGACGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 863 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 284 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTCTGGCAGATTTAGCCG |
| Suggested primer 2: | CATCGAACTGTTACCGCTGA |