| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.216899 |
| Chromosome: | chromosome 10 |
| Location: | 2395453 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435300 | AAP1 | Aspartyl aminopeptidase-like protein; (1 of 1) 3.4.11.21 - Aspartyl aminopeptidase | 3'UTR|outside_mRNA |
| Cre10.g435350 | ESU1,ESU1a | Endosulfine cAMP-regulated phosphoprotein; (1 of 2) PF04667 - cAMP-regulated phosphoprotein/endosulfine conserved region (Endosulfine) | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTCAGAGGCTATAATCGGGACGAACGGT |
| Internal bar code: | GCGCCGTCCGTCCCCTAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1046 |
| LEAP-Seq percent confirming: | 98.666 |
| LEAP-Seq n confirming: | 6213 |
| LEAP-Seq n nonconfirming: | 84 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCAATCAAGGACGTTTC |
| Suggested primer 2: | TTTTGGAGCAAGCAGAGGTT |