| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216904 |
| Chromosome: | chromosome 5 |
| Location: | 443093 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232600 | (1 of 40) 3.4.24.38 - Gametolysin / Lysin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCTTCCTGTCAGCGTGTGTCGGCTGTGC |
| Internal bar code: | CGGCAGGAATCAGCAAAGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 54 |
| LEAP-Seq percent confirming: | 75.3846 |
| LEAP-Seq n confirming: | 392 |
| LEAP-Seq n nonconfirming: | 128 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCCTCTGCCTGTACTTC |
| Suggested primer 2: | TCGTGTCTTTGGCTCTTCCT |