Insertion junction: LMJ.RY0402.216919_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.216919
Chromosome:chromosome 4
Location:495402
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g217930 (1 of 1) PF06911 - Senescence-associated protein (Senescence) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACGACATTACGCTTCATCCAGGTGGG
Internal bar code:GTTGGAGAGGGAAATCAGGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:591
LEAP-Seq percent confirming:98.7292
LEAP-Seq n confirming:3263
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:AAACCCATTTCTTGACGTGC
Suggested primer 2:ACCCTCTACAACCCAGCCTT