| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.216950 |
| Chromosome: | chromosome 1 |
| Location: | 4100666 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026900 | SSA14 | Kynurenine 3-monooxygenase; (1 of 5) 1.14.13.9 - Kynurenine 3-monooxygenase / Kynurenine 3-hydroxylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATGGTCTACCGTCTTCCTTAACTAATCA |
| Internal bar code: | TAGCGGTTTCAGATCAGATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 381 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 265 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAATCGCACACACCCTAA |
| Suggested primer 2: | TGCCCTGTCAACCTGTCATA |