| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.216957 |
| Chromosome: | chromosome 6 |
| Location: | 5007956 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280100 | (1 of 1) K08371 - cytochrome b-561 domain containing protein 2 (CYB561D2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTGGTTGCGCAACCTCCTGGACAGGGT |
| Internal bar code: | ACCGCGCCGGGGAGTTCATTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 813 |
| LEAP-Seq percent confirming: | 99.6083 |
| LEAP-Seq n confirming: | 4069 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGAATGATGCCGTTCAT |
| Suggested primer 2: | AAATGGATAGCGGCAATGAG |