Insertion junction: LMJ.RY0402.216966_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.216966
Chromosome:chromosome 10
Location:3410081
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre10.g444450 SSA17 cilia-sensing, structure and/or assembly; (1 of 1) K16462 - centrosomal protein CEP164 (CEP164) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGCGATGCAGCGGCTGTGTCTTTGCTCCC
Internal bar code:CAGGAGTGTGTATGTCTTCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:784
LEAP-Seq percent confirming:99.8473
LEAP-Seq n confirming:4578
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCGGGGTTCAGTAACTCT
Suggested primer 2:GAGGGCCAAATCGTTAAACA