Insertion junction: LMJ.RY0402.216973_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.216973
Chromosome:chromosome 17
Location:3106646
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g721250 QilinDyf-3,FAP22,IFT38,Cluap1 (1 of 1) PF10234 - Clusterin-associated protein-1 (Cluap1); Intraflagellar Transport Protein 38 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGCGGCAGCAGATGCACGCGCCACCGAC
Internal bar code:GATCTGCGTTACTCATGGTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:576
LEAP-Seq percent confirming:52.6431
LEAP-Seq n confirming:5995
LEAP-Seq n nonconfirming:5393
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTACCTGGACAGGTTCCG
Suggested primer 2:AACTCTGTGACCATCCCTCG