| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.216973 |
| Chromosome: | chromosome_17 |
| Location: | 3106646 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g721250 | FAP22 | Flagellar Associated Protein, qilin-like | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGGCGGCAGCAGATGCACGCGCCACCGAC |
| Internal bar code: | GATCTGCGTTACTCATGGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 576 |
| LEAP-Seq percent confirming: | 52.6431 |
| LEAP-Seq n confirming: | 5995 |
| LEAP-Seq n nonconfirming: | 5393 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTACCTGGACAGGTTCCG |
| Suggested primer 2: | AACTCTGTGACCATCCCTCG |