| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.216978 |
| Chromosome: | chromosome 9 |
| Location: | 3949913 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392505 | (1 of 4) PTHR22849//PTHR22849:SF39 - WDSAM1 PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCGCCGGCACTGCCGCCTGCTGCCGCA |
| Internal bar code: | TATGGTCGGAAGAGTTGCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 990 |
| LEAP-Seq percent confirming: | 83.311 |
| LEAP-Seq n confirming: | 2491 |
| LEAP-Seq n nonconfirming: | 499 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTATTTATTGCCTCGCTGC |
| Suggested primer 2: | AGATTTGGAACCACACTCCG |