Insertion junction: LMJ.RY0402.216980_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.216980
Chromosome:chromosome 3
Location:4315341
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g174850 CGL111 (1 of 1) PTHR19308:SF8 - STAR-RELATED LIPID TRANSFER PROTEIN 7, MITOCHONDRIAL; lipid-binding START protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAAGGGGAAGCGGCGCGTCCAGCGCACC
Internal bar code:CCGCTTCCTATCACGGGTAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1045
LEAP-Seq percent confirming:99.6737
LEAP-Seq n confirming:6414
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCACTTGTCTTCGGTCT
Suggested primer 2:CAAGCGACTCATCCTGCATA