| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.216987 |
| Chromosome: | chromosome 2 |
| Location: | 6728580 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118200 | (1 of 3) PF01940 - Integral membrane protein DUF92 (DUF92) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACAGGCTGGCCTTGCTCAAGCCTCGCCG |
| Internal bar code: | GCGAACGGCGGTTTTCTCCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 294 |
| LEAP-Seq percent confirming: | 98.2249 |
| LEAP-Seq n confirming: | 166 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGGTCTGCGTGTTTCAG |
| Suggested primer 2: | GCAGAGAAAGCGAGGAGAGA |