| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.217000 |
| Chromosome: | chromosome 2 |
| Location: | 3185305 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095068 | (1 of 1) PTHR15535//PTHR15535:SF18 - TRANSMEMBRANE PROTEIN 2-RELATED // COMMUNICATION MUTANT PROTEIN F-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGTGGACGTTGTACGACGGCACAAGGT |
| Internal bar code: | AAAGTTCGGCCGGCGCTCAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 312 |
| LEAP-Seq percent confirming: | 99.666 |
| LEAP-Seq n confirming: | 15516 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACGGCTGCTATGTGAGTC |
| Suggested primer 2: | ATACACACGTGCCTCCCTTC |