Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.217047 |
Chromosome: | chromosome 2 |
Location: | 6823372 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g119100 | CER2 | (1 of 2) K15889 - prenylcysteine alpha-carboxyl methylesterase (PCME); Carboxyesterase-related protein/arylformamidase | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAATCAATTGTGGGAAAGTCGGTAGGAA |
Internal bar code: | TGGAGCTGAACTTGTTCGGTCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 416 |
LEAP-Seq percent confirming: | 99.164 |
LEAP-Seq n confirming: | 2135 |
LEAP-Seq n nonconfirming: | 18 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCGCACTTCAAGATGCTGTA |
Suggested primer 2: | CTGTGTCCTCTGCCATCATC |