Insertion junction: LMJ.RY0402.217047_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.217047
Chromosome:chromosome 2
Location:6823372
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g119100 CER2 (1 of 2) K15889 - prenylcysteine alpha-carboxyl methylesterase (PCME); Carboxyesterase-related protein/arylformamidase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGAATCAATTGTGGGAAAGTCGGTAGGAA
Internal bar code:TGGAGCTGAACTTGTTCGGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:416
LEAP-Seq percent confirming:99.164
LEAP-Seq n confirming:2135
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCACTTCAAGATGCTGTA
Suggested primer 2:CTGTGTCCTCTGCCATCATC