| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217053 |
| Chromosome: | chromosome 10 |
| Location: | 935964 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424600 | (1 of 1) PF14771 - Domain of unknown function (DUF4476) (DUF4476) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCCTTCCCCGCCTCCTGCCCCATCTCC |
| Internal bar code: | GTGTCGCCATTAGGCTCCTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 713 |
| LEAP-Seq percent confirming: | 98.5714 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACCATCTTCGCAAATCC |
| Suggested primer 2: | ATGACTGGTAAGGGTGACGC |