| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.217080 |
| Chromosome: | chromosome 5 |
| Location: | 629093 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232002 | ACX2 | Acyl-CoA oxidase/dehydrogenase; (1 of 2) PTHR10909//PTHR10909:SF317 - ELECTRON TRANSPORT OXIDOREDUCTASE // ACYL-COENZYME A OXIDASE 2, PEROXISOMAL | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGAAAGGCTTGCCTGCGCGGTTCGGAGG |
| Internal bar code: | AGTAAAATGGTCGCCCACGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 522 |
| LEAP-Seq percent confirming: | 99.5854 |
| LEAP-Seq n confirming: | 1201 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAAGAGCTGCCAGTTATC |
| Suggested primer 2: | GCTATCCAGAACGCAAAAGC |