Insertion junction: LMJ.RY0402.217102_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.217102
Chromosome:chromosome 9
Location:1845758
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g395750 (1 of 6) 3.6.3.43 - Peptide-transporting ATPase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTCTGTATACTTTGCGATTCATAAAGAC
Internal bar code:GTGGCTGAGTTTTAGGTAAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:339
LEAP-Seq percent confirming:99.7314
LEAP-Seq n confirming:4085
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGCGTGTTCTTGTTCTGA
Suggested primer 2:CAACGTGGTGGTTCATTGAG