| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217124 |
| Chromosome: | chromosome 16 |
| Location: | 7128403 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680566 | (1 of 2) K17506 - protein phosphatase 1L [EC:3.1.3.16] (PPM1L, PP2CE) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTACGCCAGATGTCGCCTGCAGTGAAC |
| Internal bar code: | TTTGCACTATTACTTTTAAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1157 |
| LEAP-Seq percent confirming: | 98.9741 |
| LEAP-Seq n confirming: | 6946 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGACGTAATGACGTTTGGA |
| Suggested primer 2: | GTACGAGGGCTAGCGATGAG |