Insertion junction: LMJ.RY0402.217139_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.217139
Chromosome:chromosome 1
Location:6786037
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g048900 GTF1 Global Transcription factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCACCGCGTAGCTCCGCCTGCGTGTGGC
Internal bar code:TTGCAACGGGGTACTCTCTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:474
LEAP-Seq percent confirming:97.2863
LEAP-Seq n confirming:8138
LEAP-Seq n nonconfirming:227
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTGGGACATCGGGTAAAA
Suggested primer 2:TCACCGACTTCTTCCCAGAC