Insertion junction: LMJ.RY0402.217147_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.217147
Chromosome:chromosome 6
Location:6756444
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g294700 DIV152 (1 of 6) PF01477 - PLAT/LH2 domain (PLAT) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGCCGTCCAGCGACCTGAAGGTACCATG
Internal bar code:GCATGGTCGTGCGTACCCGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:317
LEAP-Seq percent confirming:99.8879
LEAP-Seq n confirming:8913
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTGACCTGGAGGTGAAGGTG
Suggested primer 2:ACGCAAACGCACAAGTACAA