| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.217153 |
| Chromosome: | chromosome 3 |
| Location: | 5533883 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186150 | (1 of 1) PF07047 - Optic atrophy 3 protein (OPA3) (OPA3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAATCAGGTTCTCCCCGCGCTTGCTGAC |
| Internal bar code: | GCGTTCGACGTACGTTACGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 741 |
| LEAP-Seq percent confirming: | 99.8396 |
| LEAP-Seq n confirming: | 4357 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACATGCCTGGAGTAGCAG |
| Suggested primer 2: | GCCAGCTCCACACTCTTTTC |