| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.217158 |
| Chromosome: | chromosome 6 |
| Location: | 5495638 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g284750 | EFG3 | (1 of 1) K03267 - peptide chain release factor subunit 3 (ERF3, GSPT); Translation elongation factor Tu family protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAGGTGTTGCAAACGAGCAAGTTTGCAC |
| Internal bar code: | AGTGAATACCGGAATGCACGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 917 |
| LEAP-Seq percent confirming: | 99.4781 |
| LEAP-Seq n confirming: | 18678 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTACCGAGCACTCGCAGAT |
| Suggested primer 2: | CACGACCAGTTGCTACCAGA |