| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217169 |
| Chromosome: | chromosome 6 |
| Location: | 8832265 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g310450 | (1 of 1) K03124 - transcription initiation factor TFIIB (TFIIB, GTF2B, SUA7, tfb) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTGTTGCAGAGGTACATTGCAAACGGC |
| Internal bar code: | TGTCTCCAGAACCCGGTGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1066 |
| LEAP-Seq percent confirming: | 99.7328 |
| LEAP-Seq n confirming: | 14928 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCCGACAACTGTAACAC |
| Suggested primer 2: | GTTCATGGCAACATCACGAC |