| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.217188 |
| Chromosome: | chromosome 4 |
| Location: | 1800759 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g211950 | ELG32 | Exostosin-like glycosyltransferase 32; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAACCCAACTGAACCAGGGGAGGTGAA |
| Internal bar code: | GCAATCAGGAGTTACTGTTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 326 |
| LEAP-Seq percent confirming: | 99.2727 |
| LEAP-Seq n confirming: | 10373 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTATGCAAACTTGTGCCA |
| Suggested primer 2: | GTGGAAGCTTGTCCCAACAT |