| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.217197 |
| Chromosome: | chromosome_17 |
| Location: | 5147712 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g735350 | FAP164 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCAGGGTTGGGAGGGGTGGCGCGCAGCGG |
| Internal bar code: | ACACTTGGGCGTCCAAACGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 424 |
| LEAP-Seq percent confirming: | 93.2127 |
| LEAP-Seq n confirming: | 412 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCTTATGCATGTCGTGA |
| Suggested primer 2: | TCTTGGGACTGTGTTAGGGG |