| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217204 |
| Chromosome: | chromosome 12 |
| Location: | 2582139 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505250 | FAP288 | (1 of 3) PF13202//PF13499 - EF hand (EF-hand_5) // EF-hand domain pair (EF-hand_7); EF Hand Containing Flagellar Associated Protein 288 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACACCCTGCACGGCGTGGGGGGACCCC |
| Internal bar code: | CCTACGGGCCATGCTAGTGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1148 |
| LEAP-Seq percent confirming: | 98.125 |
| LEAP-Seq n confirming: | 157 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCACCCACAACTTGAATG |
| Suggested primer 2: | AGGTTGGAGAACGGGATCTT |