| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.217239 |
| Chromosome: | chromosome 1 |
| Location: | 922263 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004950 | (1 of 2) 4.2.1.130 - D-lactate dehydratase / Glyoxylase III; Conserved Protein with Putative intracellular protease/amidase domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGAGCGCTCGCCGCTGCCGCTGCTGCGT |
| Internal bar code: | CGGGGTTTCTCTCAGAGGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 404 |
| LEAP-Seq percent confirming: | 99.1095 |
| LEAP-Seq n confirming: | 6121 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACTGTGTCCTTACGGGAT |
| Suggested primer 2: | AGTGTCACAGGGTCTACGGG |