| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217247 |
| Chromosome: | chromosome 10 |
| Location: | 4189330 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449901 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGTCACAGTCCTACCATCCAGTTGCCG |
| Internal bar code: | CCGGTGCTGGCGTTCGCTAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1011 |
| LEAP-Seq percent confirming: | 98.3868 |
| LEAP-Seq n confirming: | 11344 |
| LEAP-Seq n nonconfirming: | 186 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTTCTGCTCCTGTTTCC |
| Suggested primer 2: | TCTTGTACTTGTCGGGGGAG |