| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217252 |
| Chromosome: | chromosome 14 |
| Location: | 200778 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609030 | (1 of 3) 3.5.5.1 - Nitrilase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGCCGACTACTCGCCATGCACCCCAGT |
| Internal bar code: | GCCTAATCTTGCAGAATCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 104 |
| LEAP-Seq percent confirming: | 99.8689 |
| LEAP-Seq n confirming: | 762 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGGTGGCGGTTAATCTT |
| Suggested primer 2: | GCGACAGAGAGGAGATTTGC |